rhtrail recombinant human trail  (Merck KGaA)

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 1. rhTRAIL exposure effect alone and in combination with WT HSV-1 virus on HNSCC cell lines and the normal cell line, HaCaT. Treatment was performed with rhTRAIL at 100 ng/mL and in combination with WT HSV-1 virus at MOI 0f. 0.2 and 1.0. Cell viability analysis after 48 (A) and 72 h (B) of exposure. Results are expressed by mean percentage ± standard deviation of cell viability relative to PBS 1X (considering 100% viability). Data represent the average of three independent assays performed in triplicate. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by non-normal distribution Kruskal–Wallis test.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Virus, Standard Deviation, Comparison

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 2. Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 1.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Expressing, Standard Deviation, Control, Comparison

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 3. Expression analysis of apoptosis-associated proteins in the UD-SCC-2 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 2.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Expressing, Standard Deviation, Control, Comparison

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 4. Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 48 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of two independent assays. The asterisks indicate statistical significance (*P < 0.05) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 3.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Expressing, Standard Deviation, Control, Comparison

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 5. Expression analysis of apoptosis-associated proteins in the UD-SCC-2 cell line after 48 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the portion of total protein (non- cleaved). Data represent the average of two independent assays. The asterisks indicate statistical significance (*P < 0.05) in the comparison with the experimental groups by one-way ANOVA. Raw blots are presented in Supplementary Data 4.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Expressing, Standard Deviation, Control, Comparison

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 6. Flow cytometry apoptosis analysis after treatment with rhTRAIL ligand, WT HSV-1 and in combination. A and B) Analysis of HCB289 cell line after 24 and 48 h of treatment exposure. C and D) Analysis of UD-SCC-2 cell line after 24 and 48 h of treatment exposure. Results are expressed as mean percentage ± standard deviation of the cell death rate. Data represent the average of three independent assays. The asterisks indicate statistical significance (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001) in the comparison with the experimental groups by one-way ANOVA.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Flow Cytometry, Standard Deviation, Comparison

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 7. DR-5 expression analysis in the HCB289 cell line after treatment with rhTRAIL ligand, WT-HSV-1 and combination. (A) and (B) Detection and quantification of the receptor after 24 h of exposure treatment. (C) and (D) Detection and quantification of the receptor after 48 h of exposure treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the protein α-tubulin. Data represent the average of two independent. Raw blots are presented in Supplementary Data 5.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Expressing, Standard Deviation, Control

Journal: Scientific reports

Article Title: Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines.

doi: 10.1038/s41598-023-44888-9

Figure Lengend Snippet: Figure 8. DR-5 expression analysis in the UD-SCC-2 cell line after treatment with rhTRAIL ligand, WT-HSV-1 and combination. (A) and (B) Detection and quantification of the receptor after 24 h of exposure treatment. (C) and (D) Detection and quantification of the receptor after 48 h of exposure treatment. Results are expressed as mean percentage ± standard deviation of relative expression of cleaved protein relative to control (considering 100% expression) and normalized by the protein α-tubulin. Data represent the average of two independent assays. Raw blots are presented in Supplementary Data 6.

Article Snippet: :(0123456789) Scientific Reports | (2023) 13:18023 | https://doi.org/10.1038/s41598-023-44888-9 treated with rhTRAIL (#375-TL, R&D Systems, Minneapolis, MN, EUA) at 100 ng/mL, HSV-1 MOI 0.2 and/ or HSV-1 MOI 1.

Techniques: Expressing, Standard Deviation, Control

Journal: Molecular Therapy Oncolytics

Article Title: Molecular characterization of type I IFN-induced cytotoxicity in bladder cancer cells reveals biomarkers of resistance

doi: 10.1016/j.omto.2021.11.006

Figure Lengend Snippet: IFNα is cytotoxic to a subset of bladder cancer cell lines in multiple contexts (A) Schematic of adenoviral tool vectors. The Ad-IFNα2b vector includes hIFNα2b driven by a CMV promoter inserted into the E1 region and GFP driven by the RSV promoter inserted into the E3 region. The Ad-GFP vector includes an empty E1 region and GFP driven by the RSV promoter inserted into the E3 region. Both tool viruses lack viral E1 and E3. (B–G) Cells were exposed to (B) 100,000 U/mL rhIFNα2b, (C) 50 ng/mL TRAIL, (D) Ad-IFNα2b (MOI = 100), (E) Ad-GFP (MOI = 100) and conditioned media (CM) produced by (F) Ad-IFNα2b or (G) Ad-GFP transduced SV-HUC-1 cells. Cytotoxicity is reported as the relative fraction of dead cells after 48 h of treatment. Results are presented as mean ± SD from independent experiments performed in triplicate; N = 2 or 3 for Ad-GFP, N = 3 for all other treatments. (H) Pairwise correlation analysis was performed for all treatments and the Pearson correlation coefficient was determined. Bold indicates P < 0.05. (I) Cells were exposed to Ad-IFNα2b, Ad-GFP, or Ad-empty (MOI = 100 for all); cytotoxicity is reported as the relative fraction of dead cells after 48 h of treatment. Ad, adenovirus vector; CMV, cytomegalovirus; GFP, green fluorescent protein; rhIFNα2b, recombinant human interferon alpha 2b, rhTRAIL, recombinant human TNF-related apoptosis-inducing ligand; RSV, respiratory syncytial virus; SV40 Poly(A), simian virus 40 polyadenylated tail.

Article Snippet: The following other reagents were used in this study: rhIFNα2b (Stem Cell Technologies, 78,077), palbociclib (Cayman, 16,273), rhTRAIL (R&D 375-TL).

Techniques: Plasmid Preparation, Produced, Recombinant, Virus